Journal: Pharmaceutical Biology

Article Title: Esculin protects against methionine choline-deficient diet-induced non-alcoholic steatohepatitis by regulating the Sirt1/NF- κ B p65 pathway

doi: 10.1080/13880209.2021.1945112

Figure Lengend Snippet: Esculin attenuates MCD diet-induced fibrosis and inflammation. (A) Representative Masson trichrome staining images of the liver section from each group (400× magnification, scale bar: 50 µm). (B) Representative images from the immunohistochemistry analysis of the collagen 4A1 protein in the liver of each group (400× magnification, scale bar: 50 µm). (C) Representative immunohistochemical images of the fibronectin protein in the liver of each group (400× magnification, scale bar: 50 µm). (D) Representative immunohistochemical images of the F4/80 protein in the liver of each group (400× magnification, scale bar: 50 µm). (E) Level of TNF-α in the liver tissue. (F) Level of IL-6 in the liver tissue. (G) Level of IL-1β in the liver tissue. Data are expressed as mean ± SEM, n = 3. *** p < 0.001 vs. MCS group. # p < 0.05, ## p < 0.01, ### p < 0.001 vs. MCD group. MCS: methionine and choline supplemented; MCD: methionine and choline-deficient.

Article Snippet: The anti-fibronectin, anti-collagen 4A1, and anti-β-actin antibodies, and the TNF-α, IL-6, and IL-1β ELISA kits were purchased from Boster Biological Technology, Ltd. (Wuhan, China).

Techniques: Staining, Immunohistochemistry, Immunohistochemical staining

Journal: Pharmaceutical Biology

Article Title: Esculin protects against methionine choline-deficient diet-induced non-alcoholic steatohepatitis by regulating the Sirt1/NF- κ B p65 pathway

doi: 10.1080/13880209.2021.1945112

Figure Lengend Snippet: Sirt1 silencing affects the activity of esculin on FFA-induced inflammation and fibrosis. (A) Western blot analysis of NF-κB p65, ac-NF-κB p65, fibronectin, collagen 4A1, and β-actin. (B) Densitometric analyses of NF-κB p65. (C) Densitometric analyses of ac-NF-κB p65. (D) Densitometric analyses of fibronectin. (E) Densitometric analyses of collagen 4A1. (F) Relative NADPH/NADP + ratio. Data are expressed as mean ± SEM, n = 3. ** p < 0.01, *** p < 0.001 vs. control group. # p < 0.05, ## p < 0.01 vs. FFA group. $ p < 0.05 vs. FFA + esculin + Si-NC group. FFA: free fatty acid, NC: negative control.

Article Snippet: The anti-fibronectin, anti-collagen 4A1, and anti-β-actin antibodies, and the TNF-α, IL-6, and IL-1β ELISA kits were purchased from Boster Biological Technology, Ltd. (Wuhan, China).

Techniques: Activity Assay, Western Blot, Control, Negative Control